Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented.This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus.Significantly less time and space are required than BELLING Kensington 90 cm Electric Induction Range Cooker Red Chrome 444444051 in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps.The Sport Canteen protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.